Journal: Veterinary Pathology

Article Title: Flow cytometric analysis of equine bronchoalveolar lavage fluid cells in horses with and without severe equine asthma

doi: 10.1177/03009858211042588

Figure Lengend Snippet: Optimized concentrations of antibody for flow cytometric evaluation of cells in equine bronchoalveolar lavage fluid.

Article Snippet: The fluorescent primary antibodies used in this study were phycoerythrin (PE) conjugated mouse anti-human CD206 (clone 3.29B1, Beckman Coulter), fluorescein isothiocyanate (FITC) conjugated mouse anti-horse CD5 (clone CVS5, Bio-Rad), and PE conjugated mouse anti-horse PanB cells (clone CVS36, Bio-Rad).

Techniques: Concentration Assay

Journal: Veterinary Pathology

Article Title: Flow cytometric analysis of equine bronchoalveolar lavage fluid cells in horses with and without severe equine asthma

doi: 10.1177/03009858211042588

Figure Lengend Snippet: Flow cytometric analysis of equine bronchoalveolar lavage fluid (BALF) leukocytes. (a) Indicates that cells from p1 have higher forward scatter (FSC) and side scatter (SSC) than cells from p2, indicating they are larger and more internally complex. (b) Unstained control sample showing that many (red circle) but not all cells from p1 (upper panel) have autofluorescence in the phycoerythrin (PE) and fluorescein isothiocyanate (FITC) channels. However, cells from p2 (lower panel) are not autofluorescent. (c) Using anti-CD90 (neutrophil marker) and anti-CD206 (macrophage marker) antibodies, cells from p1 (upper panel) but not p2 (lower panel) are identified as neutrophils (Q3) and macrophages (Q1), respectively. (d) Using antibodies against CD5 (lymphocyte marker) and PanB cells, cells in p1 (upper panel) are double-negative, and cells in p2 (lower panel) are identified as lymphocytes; the red circle indicates autofluorescent cells.

Article Snippet: The fluorescent primary antibodies used in this study were phycoerythrin (PE) conjugated mouse anti-human CD206 (clone 3.29B1, Beckman Coulter), fluorescein isothiocyanate (FITC) conjugated mouse anti-horse CD5 (clone CVS5, Bio-Rad), and PE conjugated mouse anti-horse PanB cells (clone CVS36, Bio-Rad).

Techniques: Control, Marker

Journal: Stem Cell Research & Therapy

Article Title: Comparative evaluation of the therapeutic efficacy between human amniotic epithelial cells and human umbilical cord mesenchymal stem cells in premature ovarian insufficiency

doi: 10.1186/s13287-025-04881-7

Figure Lengend Snippet: Characterization of hAECs and hUC-MSCs. A Schematic illustration of the experimental design for comparing the effects of hAECs and hUC-MSCs on ovarian function. B , C Morphological feature and flow cytometric analysis of hAECs and hUC-MSCs. D , E Representative images of double immunofluorescence staining for OCT4 and CK18 or N-cadherin in hAECs and hUC-MSCs. F Multilineage differentiation potential of hUC-MSCs into osteoblasts, adipocytes, and chondrocytes, as assessed by Alizarin Red, Oil Red O, and Alcian Blue staining, respectively. Scale bars represent 25, 50, 100 and 200 μm

Article Snippet: After permeabilization with 0.1% Triton X-100 (Sigma-Aldrich, St. Louis, MO, USA), cell were incubated with the following primary antibodies at 4 °C for overnight: OCT4 (1:200, Boster), CK18 (1:200, Boster) and N-cadherin (1:200, Boster).

Techniques: Double Immunofluorescence Staining, Staining