Journal: Molecular Medicine Reports

Article Title: Heme oxygenase 1‑overexpressing bone marrow mesenchymal stem cell‑derived exosomes suppress interleukin‑1 beta‑induced apoptosis and aging of nucleus pulposus cells

doi: 10.3892/mmr.2025.13481

Figure Lengend Snippet: Activation of the NF-κB signalling in NP cells induced by IL-1β was repressed after Exo-HO-1 treatment. Western blotting was performed to detect p-p65 and/or p65 protein levels in NP cells with different treatments (control, IL-1β, IL-1β + Exo and IL-1β + Exo-HO-1) as well as in the nuclear (N) and cytoplasmic (C) fractions of the cells. Histone H3 was used as an internal reference for the nucleus. ***P<0.001 vs. control; # P<0.05, ## P<0.01 and ### P<0.001 vs. IL-1β. NP, nucleus pulposus; IL, interleukin; Exo, exosomes; HO-1, heme oxygenase 1; p-phosphorylated.

Article Snippet: After blocking with a rapid blocking solution (Beyotime Institute of Biotechnology) at 4°C overnight, the membranes were incubated with appropriate primary antibodies including anti-HO-1 (cat. no. GTX637432; 1:2,000; GeneTex, Inc.), anti-TGS101 (cat. no. A01233-2, 0.25 μg/ml; Boster Bio), anti-CD63 (FNab01490; 1:1,000; Fine Biotech Co., Ltd, Wuhan, China) and anti-Cainexin (cat. no. GTX109669; 1:5,000; GeneTex, Inc.), Bcl-2 (cat. no. FNab00839; 1:1,000; Wuhan Fine Biotech Co., Ltd.), Bax (cat. no. FNab00810; 1:2,000; Wuhan Fine Biotech Co., Ltd.), cleaved caspase 3 (cat. no. MBS9410752; 1:1,000; MyBioSource, Inc.), anti-p65 (cat. no. PB9324; 0.5 μg/ml; Boster Bio), anti-p-p65 (cat. no. A00284S468-2; 1:2,000; Boster Bio), anti-GAPDH (cat. no. H00227; 1:5,000; Boster Bio) and anti-Histone H3 (cat. no. M12477-9; 1:1,000; Boster Bio) antibodies.

Techniques: Activation Assay, Western Blot, Control

Journal: Immunity, Inflammation and Disease

Article Title: VEGF-C improves regeneration and lymphatic reconnection of transplanted autologous lymph node fragments: An animal model for secondary lymphedema treatment

doi: 10.1002/iid3.32

Figure Lengend Snippet: Primary and secondary antibodies used

Article Snippet: B-lymphocytes , BM 4013 , Acris antibodies, Herford, Germany , Mouse , ×.

Techniques:

Journal: Immunity, Inflammation and Disease

Article Title: VEGF-C improves regeneration and lymphatic reconnection of transplanted autologous lymph node fragments: An animal model for secondary lymphedema treatment

doi: 10.1002/iid3.32

Figure Lengend Snippet: H&E staining and immunohistochemistry. (a and b) H&E staining of a necrotic (a) and a potential regenerated (b) transplant. (c–i) Regenerated transplant after immunohistochemistry. (c–e) Primary antibodies used against T-lymphocytes (R73) and B-lymphocytes (BM 4013) and immunofluorescent secondary antibodies FITC and AF564. (c) T-lymphocytes are located paracortically. (d) B-lymphocytes are located cortically. (e) Merged image. (f and g) Primary antibody used against lymphatic endothelial cells (LYVE-1) and immunofluorescent secondary antibody Cy3. (h and i) Primary antibody used against high endothelial venules (His52) and immunofluorescent secondary antibody AF546. H&E, hematoxylin and eosin.

Article Snippet: B-lymphocytes , BM 4013 , Acris antibodies, Herford, Germany , Mouse , ×.

Techniques: Staining, Immunohistochemistry